对具有高转糖基化活性的试剂JA300的研究。

Study of JA300 as agent having high transglycosylation activity.

作者信息

Izumi M, Kojima E, Ishida S, Kaneko R, Miyawaki S, Kawaoka A, Machida M, Murakami K

机构信息

Biochemistry Section, Iwakuni Research Laboratory of Technology, Nippon Paper Industries Co. Ltd., Yamaguchi, Japan.

出版信息

Nucleic Acids Symp Ser. 1995(34):105-6.

PMID:8841574

Abstract

Escherichia coli JA300 as the strain catalyzing transglycosylation of nucleic acids has so strong activity. We scrutinized where the high faculty of this strain come from. Using PCR methods with our primer pair MI-1 (up; GTT GAA TTC GCC TTT GTT ATG TCA C) and MI-2 (down; GTT CCG CTA ACG GAA CAC CTA GGC CT), we isolated deoD (the coding region of PNPase) of JA300. Nucleotide sequence analysis indicated that deoD of JA300 is definitely same as that of parent strain K12. The fact that the transcription of deoD of JA300 is more active than that of K12 was also revealed after the analysis by Northrn blot hybridization. These data may suggest that the varieties of gene concerning regulation contribute to the difference of PNPase activity between them.

摘要

作为催化核酸转糖基化的菌株，大肠杆菌JA300具有很强的活性。我们研究了该菌株这种高活性的来源。使用我们的引物对MI-1（上游；GTT GAA TTC GCC TTT GTT ATG TCA C）和MI-2（下游；GTT CCG CTA ACG GAA CAC CTA GGC CT）通过PCR方法，我们分离出了JA300的deoD（PNPase的编码区）。核苷酸序列分析表明JA300的deoD与亲本菌株K12的deoD完全相同。通过Northern印迹杂交分析后还发现，JA300的deoD转录比K12的更活跃。这些数据可能表明，与调控相关的基因差异导致了它们之间PNPase活性的不同。