Tomita R, Morikawa Y, Hisamatsu S, Suzuki-Fujita K, Tanaka T, Sonoki S, Kikuchi Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Nucleic Acids Symp Ser. 1995(34):117-8.
We have previously shown that the hairpin ribozyme-like structure of the negative strand of the satellite RNA of arabis mosaic virus [(- )sArMV] has indeed ribozyme activity. However, some mutagenesis analyses revealed surprisingly that the wild type ribozyme was less active than almost all the other mutant ribozymes tested. These results were derived from a trans-acting system. Here we tested this ribozyme activity in a cis-acting system. We show that the (-)s ArMV hairpin ribozyme has different target-site specificities between cis and trans cleavages. The wild type ribozyme has the highest self-cleaving activity among the ribozyme variants tested.
我们之前已经表明，阿拉伯花叶病毒卫星RNA负链[（-）sArMV]的发夹状核酶样结构确实具有核酶活性。然而，一些诱变分析令人惊讶地发现，野生型核酶的活性低于几乎所有测试的其他突变核酶。这些结果来自于反式作用系统。在这里，我们在顺式作用系统中测试了这种核酶活性。我们表明，（-）sArMV发夹状核酶在顺式和反式切割之间具有不同的靶位点特异性。在所测试的核酶变体中，野生型核酶具有最高的自我切割活性。
