反义寡核苷酸对流感病毒RNA聚合酶及基因表达核蛋白的抑制作用

Inhibition of influenza virus RNA polymerase and nucleoprotein of gene expression by antisense oligonucleotides.

作者信息

Hatta T, Nakagawa Y, Takai K, Nakada S, Yokota T, Takaku H

机构信息

Department of Industrial Chemistry, Chiba Institute of Technology, Japan.

出版信息

Nucleic Acids Symp Ser. 1995(34):129-30.

PMID:8841586

Abstract

We demonstrated that unmodified and modified (phosphorothioate) antisense oligonucleotides inhibit CAT (chloramphenicol acetyltransferase) protein expression in the clone 76 cell line. This cell line expresses the influenza virus RNA polymerase and nucleoprotein (NP) genes in response to dexamethasone. Antisense oligonucleotides with four target sites (PB1, PB2, PA, and NP) were synthesized and tested for the their inhibitory effects by a CAT-ELISA assay. Antisense phosphorothioate oligonucleotides (S-ODNs) complementary to the sites of the PB2-AUG and PA-AUG initiation codons showed a high inhibitory effect. On the other hand, the inhibitory effect of the S-ODNs targeted to PB1 was considerably decreased in comparison with the other three target sites.

摘要

我们证明，未修饰的和经修饰的（硫代磷酸酯）反义寡核苷酸可抑制克隆76细胞系中氯霉素乙酰转移酶（CAT）蛋白的表达。该细胞系可响应地塞米松表达流感病毒RNA聚合酶和核蛋白（NP）基因。合成了具有四个靶位点（PB1、PB2、PA和NP）的反义寡核苷酸，并通过CAT-ELISA测定法测试了它们的抑制作用。与PB2-AUG和PA-AUG起始密码子位点互补的硫代磷酸酯反义寡核苷酸（S-ODNs）显示出高抑制作用。另一方面，与其他三个靶位点相比，靶向PB1的S-ODNs的抑制作用明显降低。