Johnson M L, Ackers G K
Biophys Chem. 1977 Jun;7(1):77-80. doi: 10.1016/0301-4622(77)87017-8.
Analyses of low concentration oxygenation curves for apparent Adair constants in which the effects of dimers is ignored have been explored using recently determined values of the overall energy coupling parameters. For high affinity systems and favorable energy distributions, it is found that the errors in estimated binding free energies may be less than one kcal provided the measurement errors are strictly random and of small magnitude. These errors are nevertheless quite substantial as compared with the differences between values for the successive binding steps.
利用最近测定的总能量耦合参数值，对忽略二聚体影响的表观阿代尔常数的低浓度氧合曲线进行了分析。对于高亲和力系统和有利的能量分布，发现如果测量误差严格随机且幅度较小，估计的结合自由能误差可能小于1千卡。然而，与连续结合步骤的值之间的差异相比，这些误差相当大。
