癸基奥拉菌素C和D对兼性光合细菌呼吸电子流的影响。

The effects of decyl aurachins C and D on the respiratory electron flow of facultative phototrophic bacteria.

作者信息

Romagnoli S, Oettmeier W, Zannoni D

机构信息

Department of Biology, Univ. of Bologna, Italy.

出版信息

Biochem Mol Biol Int. 1996 Jul;39(4):671-8. doi: 10.1080/15216549600201741.

DOI:10.1080/15216549600201741

PMID:8843334

Abstract

Decyl aurachins C and D are two synthetic compounds related to the natural products aurachins C and D extracted from Stigmatella aurantiaca. Titrations of a range of partial respiratory activities in membranes from facultative phototrophs indicate that decyl aurachin C is more effective than aurachin D in inhibiting the quinol oxidase. Decyl aurachin C also affects the NADH-ubiquinone oxidoreductase of Rhodobacter capsulatus and the cytochrome bc1 complexes of Rb.capsulatus and Rhodospirillum centenum. Titration of the light-induced respiration in Rsp.centenum allowed us to estimate an upper limit for the ubiquinol oxidase concentration of 1.5 +/- 0.2 nmol/mg protein, or a ubiquinol oxidase/cytochrome c oxidase ratio of 3:1.

摘要

癸基金耳素C和D是两种与从橙色粘球菌中提取的天然产物金耳素C和D相关的合成化合物。对一系列兼性光合生物膜中的部分呼吸活性进行滴定表明，癸基金耳素C在抑制喹啉氧化酶方面比金耳素D更有效。癸基金耳素C还影响荚膜红细菌的NADH-泛醌氧化还原酶以及荚膜红细菌和中心红螺菌的细胞色素bc1复合体。对中心红螺菌中光诱导呼吸的滴定使我们能够估计泛醇氧化酶浓度的上限为1.5±0.2 nmol/mg蛋白质，或泛醇氧化酶/细胞色素c氧化酶的比例为3:1。