Zechmeister A
Histochemistry. 1979 Jun 18;61(2):223-32. doi: 10.1007/BF00496534.
A new, simple, rapid, and highly sensitive and selective method for the ultrahistochemical detection of calcium is described. The reagent N,N-Naphthaloylhydroxylamine (1,8-C10H6CON(ONa)CO) sodium salt was employed in this study for the demonstration of calcium at the subcellular level in relaxed and contracted muscles (smooth muscle of the stomach, thoracic aorta, and myocardial muscle cells) of the rat (in vitro as well as in vivo) and in the human vascular smooth muscle of the aorta with atherosclerotic calcification. Direct evidence of the presence of calcium in the electron-dense reaction products (calcium N,N-Naphthaloylhydroxylamine) is given by X-ray microanalysis of 1,500-2,000 A thick sections. The significance of distributional differences in the localization of calcium in subcellular structures of relaxed and contracted muscles is discussed in relation to the role of calcium in the control of the muscle activity during the contraction-relaxation cycle.
本文描述了一种用于超组织化学检测钙的新的、简单、快速且高度灵敏和选择性的方法。本研究采用试剂N,N-萘甲酰羟胺（1,8-C₁₀H₆CON(ONa)CO）钠盐，在大鼠（体外及体内）的松弛和收缩肌肉（胃平滑肌、胸主动脉和心肌细胞）以及有动脉粥样硬化钙化的人主动脉血管平滑肌中，在亚细胞水平上显示钙。通过对1500 - 2000埃厚切片进行X射线微分析，给出了电子致密反应产物（钙N,N-萘甲酰羟胺）中钙存在的直接证据。结合钙在收缩 - 舒张周期中对肌肉活动控制的作用，讨论了松弛和收缩肌肉亚细胞结构中钙定位分布差异的意义。
