Ukuku D O, Shelef L A
Department of Nutrition and Food Science, Wayne State University, Detroit, MI 48202, USA.
J Biolumin Chemilumin. 1996 May-Jun;11(3):169-73. doi: 10.1002/(SICI)1099-1271(199605)11:3<169::AID-BIO410>3.0.CO;2-R.
The influence of nisin on intracellular ATP and cell numbers of Listeria monocytogenes strain Scott A was determined and compared with the effect of amplicillin and streptomycin under similar conditions. In the presence of nisin (3-12 micrograms/ml), intracellular ATP and cell numbers decreased rapidly during the first hour at 35 degrees C and extracellular ATP increased. Cell numbers and intracellular ATP increased after 3 h of incubation. No effect was observed in cells treated with ampicillin (3-12 micrograms/ml) and streptomycin (15-60 micrograms/ml) during the first hour. However, concentrations of > or = 3 micrograms/ml ampicillin and > or = 30 micrograms/ml streptomycin were listeriostatic after 3 h of incubation. Progressive loss of viability and reduction of intracellular ATP were observed in resting cells in PBS (pH 7.2) containing increasing concentrations of the antimicrobials. Rapid accumulation of extracellular ATP, observed immediately after treatment with nisin but not with the antibiotics, supports the reported collapse of proton motive force in L. monocytogenes by nisin.
测定了乳酸链球菌素对单核细胞增生李斯特菌菌株Scott A细胞内ATP和细胞数量的影响，并在相似条件下与氨苄青霉素和链霉素的作用进行了比较。在存在乳酸链球菌素（3 - 12微克/毫升）的情况下，在35℃孵育的最初1小时内，细胞内ATP和细胞数量迅速下降，而细胞外ATP增加。孵育3小时后，细胞数量和细胞内ATP增加。在最初1小时内，用氨苄青霉素（3 - 12微克/毫升）和链霉素（15 - 60微克/毫升）处理的细胞未观察到影响。然而，孵育3小时后，浓度≥3微克/毫升的氨苄青霉素和≥30微克/毫升的链霉素具有抑制李斯特菌生长的作用。在含有浓度不断增加的抗菌剂的PBS（pH 7.2）中，静息细胞的活力逐渐丧失，细胞内ATP减少。在用乳酸链球菌素处理后立即观察到细胞外ATP的快速积累，而用抗生素处理则未观察到，这支持了报道的乳酸链球菌素导致单核细胞增生李斯特菌质子动力势崩溃的现象。
