Sommer L, Shah N, Rao M, Anderson D J
Division of Biology 216-76, California Institute of Technology, Pasadena 91125, USA.
Neuron. 1995 Dec;15(6):1245-58. doi: 10.1016/0896-6273(95)90005-5.
Using primary cultures and immortalized multipotential stem cell lines derived from wild-type and Mash1 mutant neural crest cells, we have analyzed the cellular function of MASH1 in autonomic neurogenesis. We present evidence for the existence of a precursor expressing MASH1 and neuronal markers such as neurofilament, neuron-specific tubulin, and tetanus toxin receptor. This cell has a nonneuronal morphology. Differentiation of this precursor to neurons that express markers such as SCG10, peripherin, and neuron-specific enolase is dependent upon MASH1 function. These data imply that the differentiation of autonomic neurons from uncommitted neural crest cells occurs in several sequential steps. Moreover, they suggest that MASH1 does not commit multipotent cells to a neural fate, like its Drosophila achaete-scute counterparts, but rather promotes the differentiation of a committed neuronal precursor.
利用源自野生型和Mash1突变神经嵴细胞的原代培养物和永生化多能干细胞系，我们分析了MASH1在自主神经发生中的细胞功能。我们提供了证据，证明存在一种表达MASH1和神经元标志物（如神经丝、神经元特异性微管蛋白和破伤风毒素受体）的前体细胞。这种细胞具有非神经元形态。这种前体细胞向表达如SCG10、外周蛋白和神经元特异性烯醇化酶等标志物的神经元的分化依赖于MASH1的功能。这些数据表明，自主神经元从不成熟神经嵴细胞的分化发生在几个连续的步骤中。此外，它们表明MASH1并不像其果蝇achaete-scute对应物那样使多能细胞确定为神经命运，而是促进已确定的神经元前体细胞的分化。
