Nogami H, Tachibana T, Katayama T, Ishikawa H
Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan.
Arch Histol Cytol. 1995 Dec;58(5):581-9. doi: 10.1679/aohc.58.581.
The effect of synthetic glucocorticoid, dexamethasone (DEX), on the fine structure of the fetal growth hormone (GH) cell was examined in rats on Days 17-19 of gestation by the protein A-gold method of immuno-electron microscopy. GH cells appeared on Day 19 in the control fetuses. The DEX-induced GH cells of Day 17 fetuses displayed more immature features than those of Day 18, which closely resembled GH cells found in the control fetus on Day 19. Cytological changes which indicate increased secretory activity such as the development of the rough endoplasmic reticulum or enlargement of the Golgi complex, and an accumulation of secretory granules were evident only in the DEX-treated fetuses on Day 19. It is concluded that DEX not only accelerates the development of GH cells, as observed on Day 17 or 18, but also brings them to a functionally stimulated state by Day 19.
采用免疫电子显微镜蛋白A-金标法，研究了合成糖皮质激素地塞米松（DEX）对妊娠17至19天大鼠胎儿生长激素（GH）细胞超微结构的影响。对照组胎儿的GH细胞在第19天出现。第17天经DEX诱导的GH细胞比第18天的表现出更不成熟的特征，后者与第19天对照组胎儿中发现的GH细胞非常相似。仅在第19天经DEX处理的胎儿中观察到表明分泌活性增加的细胞学变化，如粗面内质网的发育或高尔基体复合体的增大，以及分泌颗粒的积累。得出的结论是，DEX不仅如在第17天或18天观察到的那样加速了GH细胞的发育，而且到第19天时还使它们进入功能受刺激状态。
