Bara M, Guiet-Bara A, Moretto P, Razafindrabe L, Llabador Y, Simonoff M, Durlach J
Laboratoire de Physiopathologie du Développement, Université P.M. Curie, Paris, France.
Magnes Res. 1995 Sep;8(3):207-14.
The effect of the addition of MgCl2 on the Na+, K+, and Cl- concentration and distribution in epithelial and compact layers of the human amniotic membrane was investigated using the Bordeaux nuclear microprobe. Particle-induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological medium (Hanks' solution), the monovalent ion concentrations were identical in both layers. The addition of Mg2+ ions in Hanks' solution induced a decrease of, K+, and Cl- concentration in both layers and, Na+ concentration in the compact layer. The results obtained from nuclear microanalysis might be explained from electrophysiological data which indicate that the addition of Mg2+ ions results in an increase in the cellular, paracellular and exchanger ion pathways.
使用波尔多核微探针研究了添加MgCl₂对人羊膜上皮层和致密层中Na⁺、K⁺和Cl⁻浓度及分布的影响。采用粒子诱导X射线发射和卢瑟福背散射光谱技术进行定量测量。在生理介质（汉克斯溶液）中，两层中的单价离子浓度相同。在汉克斯溶液中添加Mg²⁺离子会导致两层中K⁺和Cl⁻浓度降低，致密层中Na⁺浓度降低。核微分析获得的结果可能用电生理数据来解释，该数据表明添加Mg²⁺离子会导致细胞、细胞旁和交换器离子途径增加。
