Sjölund B, Björklund A, Wiklund L
Brain Res. 1977 Aug 5;131(1):23-37. doi: 10.1016/0006-8993(77)90026-9.
The possible involvement of serotoninergic mechanisms in the induction of harmaline generated tremor in the inferior olive has been investigated electrophysiologically in the cat and rat. Mass recordings of Purkinje cell activity in the cat showed that harmaline induces strong, synchronous and rhythmic activity in those parts of the climbing fibre system originating in the caudal part of the medial accessory olive and the caudolateral parts of the dorsal accessory nucleus. These are the areas of the cat olive shown to receive a dense serotoninergic innervation. In the rat, the selective removal of the serotoninergic innervation--produced by an intraventricular injection of 5,6-dihydroxytryptamine, or 5,7-dihydroxytryptamine in combination with desipramine--caused a significant attenuation of both the tremor and the climbing fibre activity induced by an intravenous harmaline injection. In the 5,6-dihydroxytryptamine-treated animals the reappearance of the harmaline tremor seemed to parallel the regrowth of new serotoninergic axon sprouts in the inferior olive. On the basis of the present results it is proposed that the serotoninergic afferents to the accessory olivary nuclei are of critical importance for the tremor induction of harmaline in the inferior olive. It is suggested that harmaline, rather than acting directly on the olivary neurones, exerts its effect through an interference with a serotoninergic (possibly inhibitory) innervation of these cells.
已通过猫和大鼠的电生理学方法研究了血清素能机制在毒扁豆碱诱发下橄榄核震颤中的可能作用。对猫浦肯野细胞活动的群体记录显示，毒扁豆碱在起源于内侧副橄榄核尾部和背侧副核尾外侧部的攀缘纤维系统的那些部位诱发强烈、同步且有节律的活动。这些是猫橄榄核中显示接受密集血清素能神经支配的区域。在大鼠中，通过脑室内注射5,6 - 二羟基色胺或5,7 - 二羟基色胺与地昔帕明联合使用来选择性去除血清素能神经支配，导致静脉注射毒扁豆碱诱发的震颤和攀缘纤维活动显著减弱。在5,6 - 二羟基色胺处理的动物中，毒扁豆碱震颤的再次出现似乎与下橄榄核中新的血清素能轴突发芽的再生平行。基于目前的结果，有人提出，向副橄榄核的血清素能传入神经对毒扁豆碱在下橄榄核中诱发震颤至关重要。有人认为，毒扁豆碱并非直接作用于橄榄核神经元，而是通过干扰这些细胞的血清素能（可能是抑制性）神经支配来发挥其作用。
