Nakanishi M, Tamada Y, Deyashiki Y, Hara A
Laboratory of Biochemistry, Gifu Pharmaceutical University, Japan.
Biol Pharm Bull. 1995 Sep;18(9):1248-50. doi: 10.1248/bpb.18.1248.
Tetrameric carbonyl reductase of pig lung has been shown by cDNA cloning to be a member of the short-chain dehydrogenase family of enzymes. Construction of the carbonyl reductase cDNA in an expression vector yielded an abundant, enzymatically active enzyme in Escherichia coli. The recombinant enzyme was purified to homogeneity and shown to be structurally, immunologically and functionally similar to lung carbonyl reductase.
猪肺中的四聚体羰基还原酶经cDNA克隆已表明是短链脱氢酶家族的一种酶。将羰基还原酶cDNA构建到表达载体中，在大肠杆菌中产生了大量具有酶活性的酶。重组酶被纯化至同质，并显示在结构、免疫和功能上与肺羰基还原酶相似。
