Ramsell K D, Zhao B G, Baker D, Cobbett P
Department of Pharmacology & Toxicology, Michigan State University, East Lansing 48824, USA.
Brain Res Bull. 1996;39(2):109-14. doi: 10.1016/0361-9230(95)02057-8.
The effects of serum on the morphological plasticity exhibited by pituicytes in explant cultures of the neurohypophysis of adult rats have been examined. Cultured pituicytes are normally nonstellate, protoplasmic, amorphous cells (< 25% are stellate with a distinct cell body and phase bright processes). After incubation (90 min) of pituicyte cultures in a HEPES buffered salt solution (HBSS) supplemented with isoproterenol or forskolin, the fraction of stellate pituicytes significantly increased. The increase in the fraction of stellate cells induced by isoproterenol was not reversed by subsequent incubation in isoproterenol-free HBSS for 90 min. In contrast, after stellation was induced in cultures by exposure to forskolin (90 min), the fraction of stellate cells was significantly reduced if these cultures were incubated in forskolin-free, serum (0.5%) supplemented HBSS for the same duration. Serum also blocked the increase in the fraction of stellate pituicytes induced by forskolin. These experiments suggest that serum components may have a significant role in controlling the plasticity of neuroglial relations in the neurohypophysis previously demonstrated in vivo.
研究了血清对成年大鼠神经垂体组织块培养中垂体细胞形态可塑性的影响。培养的垂体细胞通常为非星状、原生质、无定形细胞（<25%为具有明显细胞体和亮相位突起的星状细胞）。在添加异丙肾上腺素或福斯高林的HEPES缓冲盐溶液（HBSS）中培养垂体细胞90分钟后，星状垂体细胞的比例显著增加。异丙肾上腺素诱导的星状细胞比例增加，在随后不含异丙肾上腺素的HBSS中培养90分钟后并未逆转。相反，在培养物中暴露于福斯高林90分钟诱导形成星状细胞后，如果将这些培养物在不含福斯高林、添加0.5%血清的HBSS中培养相同时间，星状细胞的比例会显著降低。血清也会阻断福斯高林诱导的星状垂体细胞比例增加。这些实验表明，血清成分可能在控制先前在体内已证明的神经垂体中神经胶质关系的可塑性方面发挥重要作用。
