从人血浆中纯化硒蛋白Ph的改进方法。
Improved procedure for the purification of selenoprotein Ph from human plasma.
作者信息
Eberle B, Haas H J
机构信息
Fachrichtung Medizinische Biochemie der Universität des Saarlandes, Homburg/Saar, Germany.
出版信息
J Trace Elem Med Biol. 1995 Mar;9(1):55-7. doi: 10.1016/S0946-672X(11)80010-6.
Selenoprotein Ph, the human analogue of selenoprotein P from rat plasma, was purified from human plasma using Heparin Sepharose chromatography, PEG precipitation, DEAE ion exchange chromatography. RP chromatography, SDS-PAGE and electroelution. SDS-PAGE of the purified protein revealed one broad-selenium containing protein band from 56 to 67 kDa with a selenium maximum at 62 kDa. Using a 7.5% T gel this band was separated into two distinct selenium-containing bands with molecular weights of 61 and 64 kDa.
硒蛋白Ph是大鼠血浆中硒蛋白P的人类类似物，通过肝素琼脂糖层析、聚乙二醇沉淀、DEAE离子交换层析、反相层析、SDS-聚丙烯酰胺凝胶电泳和电洗脱从人血浆中纯化得到。纯化蛋白的SDS-聚丙烯酰胺凝胶电泳显示出一条56至67 kDa的宽含硒蛋白带，在62 kDa处硒含量最高。使用7.5%的T凝胶，该条带被分离成两条不同的含硒带，分子量分别为61 kDa和64 kDa。
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