纤溶酶原激活剂在猪肾细胞株LLC-PK1（LP100）上的免疫细胞化学定位

Immunocytochemical localization of plasminogen activator on porcine kidney cell strain: LLC-PK1 (LP100).

作者信息

Paul D C, Bobbitt J L, Williams D C, Hull R N

出版信息

J Histochem Cytochem. 1979 Jun;27(6):1035-40. doi: 10.1177/27.6.88472.

DOI:10.1177/27.6.88472

Abstract

An antibody to plasminogen activator (PA) produced by the cultured cells of the pig kidney cell strain LLC-PK1 (LP100) was used to localize PA on the cell's free (unattached) surface. Localization was accomplished by the unlabeled antibody enzyme method (PAP) at the light microscopic level and at the electron microsopic level. Localization was commonly more intense at cell to cell junctions and was associated with blebs and vesiculation in this area. We are proposing that membrane shedding by blebs and vesiculation may be the mechanism of PA release in the LLC-PK1 (LP100) cell strain.

摘要

用猪肾细胞系LLC-PK1（LP100）培养细胞产生的纤溶酶原激活物（PA）抗体，将PA定位在细胞的游离（未附着）表面。通过未标记抗体酶法（PAP）在光学显微镜和电子显微镜水平完成定位。在细胞间连接处定位通常更强，并与该区域的泡状突起和小泡形成有关。我们提出，泡状突起和小泡形成导致的膜脱落可能是LLC-PK1（LP100）细胞系中PA释放的机制。