葡萄糖酸盐对林蛙离体单收缩肌纤维电荷移动成分的差异性抑制作用
Differential suppression of charge movement components by gluconate in cut twitch fibres of Rana temporaria.
作者信息
Hui C S, Chen W
机构信息
Department of Physiology and Biophysics, Indiana University Medical Center, Indianapolis 46202, USA.
出版信息
J Physiol. 1995 Dec 1;489 ( Pt 2)(Pt 2):511-7. doi: 10.1113/jphysiol.1995.sp021068.
Abstract
- Charge movement was studied in cut twitch fibres of Rana temporaria using a double Vaseline-gap voltage-clamp technique. 2. Replacement of Cl- by gluconate in the external solution reduced the magnitude of the early current component (I beta) substantially but affected the magnitude and slowed the kinetics of the hump current component (I gamma) slightly. 3. The early (Q beta) and hump (Q gamma) charge components in the gluconate solution were 11.8 +/- 2.3 and 88.0 +/- 4.8% (mean +/- S.E.M., n = 9), respectively, of those in the Cl- solution. 4. These results suggest that Q beta cannot be a precursor of Q gamma. Moreover, since fibres bathed in a gluconate solution can release calcium, Q beta is probably not involved in triggering calcium release.
摘要
- 使用双凡士林间隙电压钳技术研究了林蛙离体单收缩肌纤维中的电荷移动。2. 用葡萄糖酸盐替代外部溶液中的氯离子，显著降低了早期电流成分（Iβ）的幅度，但对驼峰电流成分（Iγ）的幅度有轻微影响，并使其动力学变慢。3. 在葡萄糖酸盐溶液中，早期（Qβ）和驼峰（Qγ）电荷成分分别为氯离子溶液中相应成分的11.8±2.3%和88.0±4.8%（平均值±标准误，n = 9）。4. 这些结果表明，Qβ不可能是Qγ的前体。此外，由于浸泡在葡萄糖酸盐溶液中的纤维可以释放钙，Qβ可能不参与触发钙释放。
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