Sugimura D, Fukue H, Arai M, Matsumoto K, Fukutake K
Department of Clinical Pathology, Tokyo Medical College.
Rinsho Byori. 1996 Apr;44(4):355-61.
Factor XIII is composed of two distinct proteins, a and b subunit. The dimers of each subunit form a tetrameric complex of a2b2 in plasma. Two separate ELISA systems were developed to measure either factor XIII a or b subunit in plasma. Sensitivity of the ELISAs was 3.0% of factor XIII a and b subunit in normal plasma. The ELISA for factor XIII b subunit quantitate free b2 and a2b2 complex in a equimolar manner. In order to evaluate the changes of each factor XIII subunit in a state of hypercoagulation, 16 plasmas from proteins with disseminated intravascular coagulation syndrome (DIC) were examined. Factor XIII a and b subunit showed 56.5 +/- 30.5% and 63.4 +/- 22.5%, respectively in the DIC subjects compared to normal pooled plasma as 100%. The ratio of factor XIII a and b subunit (a/b ratio) were between 0.5 and 1.0 in DIC cases which improved after treatment. However, the ratios were less than 0.5 in the cases with DIC which deteriorated in spite of the treatment. These results suggest that the a/b ratio would indicate the prognosis of patients with DIC.
因子 XIII 由两种不同的蛋白质，即 a 亚基和 b 亚基组成。每个亚基的二聚体在血浆中形成 a2b2 的四聚体复合物。开发了两种独立的 ELISA 系统来测量血浆中的因子 XIII a 或 b 亚基。ELISA 的灵敏度为正常血浆中因子 XIII a 和 b 亚基的 3.0%。因子 XIII b 亚基的 ELISA 以等摩尔方式定量游离的 b2 和 a2b2 复合物。为了评估高凝状态下每个因子 XIII 亚基的变化，检测了 16 份患有弥散性血管内凝血综合征（DIC）患者的血浆。与作为 100% 的正常混合血浆相比，DIC 患者的因子 XIII a 和 b 亚基分别显示为 56.5 +/- 30.5% 和 63.4 +/- 22.5%。在治疗后病情改善的 DIC 病例中，因子 XIII a 和 b 亚基的比例（a/b 比值）在 0.5 至 1.0 之间。然而，在尽管接受治疗但病情仍恶化的 DIC 病例中，该比值小于 0.5。这些结果表明，a/b 比值可指示 DIC 患者的预后。
