Lin L H, Wang L H
Department of Pharmacology, Chang Gung College of Medicine and Technology, Taiwan, ROC.
Neurosci Lett. 1996 Jan 5;202(3):149-52. doi: 10.1016/0304-3940(95)12227-3.
The aim of this study was to investigate whether the mRNA level of gamma-aminobutyric acid (GABA)A receptor delta subunit could be altered by chronic pentobarbital treatment. Male ICR mice were rendered tolerant to and dependent upon pentobarbital by repeated pentobarbital administration and by abrupt pentobarbital withdrawal, respectively. The levels of the delta subunit mRNA in the frontal cortex and cerebellum were quantified using RNase protection assay in the presence of the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase, as an internal standard. Our results revealed that the delta subunit mRNA in the cerebellum was upregulated in pentobarbital-tolerant mice and was downregulated in pentobarbital-withdrawn mice. No significant changes were found in the frontal cortex. These results suggest that chronic pentobarbital exposure can modify the expression of GABAA receptor delta subunit in a region-specific manner.
本研究的目的是调查慢性戊巴比妥治疗是否会改变γ-氨基丁酸（GABA）A受体δ亚基的mRNA水平。雄性ICR小鼠分别通过反复给予戊巴比妥和突然撤药，使其对戊巴比妥产生耐受性和依赖性。在持家基因甘油醛-3-磷酸脱氢酶作为内参基因存在的情况下，采用核糖核酸酶保护分析法定量额叶皮质和小脑中δ亚基mRNA的水平。我们的结果显示，在戊巴比妥耐受小鼠中，小脑中的δ亚基mRNA上调，而在撤药小鼠中则下调。额叶皮质未发现显著变化。这些结果表明，慢性戊巴比妥暴露可通过区域特异性方式改变GABAA受体δ亚基的表达。
