Kajander K C, Madsen A M, Iadarola M J, Draisci G, Wakisaka S
Department of Oral Science, University of Minnesota, Minneapolis 55455, USA.
Neurosci Lett. 1996 Mar 8;206(1):9-12. doi: 10.1016/0304-3940(96)12447-2.
A chronic constriction injury (CCI), transection injury, or sham injury to the sciatic nerve was induced in 30 rats. Rats were then sacrificed at 1, 3, 5, 10, and 20 days following injury, and the number of cells immunohistochemically labeled for Fos-like immunoreactivity (Fos-LI) was determined in random sections from the lumbar 4 and 5 (L4 and L5) spinal segments. Non-parametric statistics (Wilcoxon) were used to compare the number of labeled cells ipsilateral to the injury to the number of labeled cells on the contralateral side. At 1 and 5 days following injury, in all treatment groups, significantly more labeled cells (P < 0.05) were observed ipsilaterally. In addition, at 3 and 10 days following injury, the CCI groups exhibited significantly more labeled cells ipsilaterally. The significant increases for the CCI groups ranged from 161% to 360%. Generally, increases were greater for the CCI groups. These results indicate that Fos-LI increases to a greater extent and for a longer duration following the CCI than following either a transection or sham injury.
对30只大鼠造成坐骨神经慢性压迫损伤（CCI）、横断损伤或假损伤。然后在损伤后的1、3、5、10和20天处死大鼠，并在来自腰4和腰5（L4和L5）脊髓节段的随机切片中测定免疫组织化学标记为Fos样免疫反应性（Fos-LI）的细胞数量。使用非参数统计（Wilcoxon）比较损伤同侧标记细胞的数量与对侧标记细胞的数量。在损伤后的1天和5天，在所有治疗组中，同侧观察到的标记细胞明显更多（P<0.05）。此外，在损伤后的3天和10天，CCI组同侧的标记细胞明显更多。CCI组的显著增加范围为161%至360%。一般来说，CCI组的增加幅度更大。这些结果表明，与横断损伤或假损伤相比，CCI后Fos-LI增加的程度更大且持续时间更长。
