Influence of progesterone levels on prostaglandin concentrations in isolated uterine tissue and incubation medium from pseudopregnant rats.

We measured the synthesis and secretion of uterine prostaglandins E and F2 alpha (PGE and PGF2 alpha) by the uterus in vitro from pseudopregnant (psp) rats on days 5 and 10 of psp, when level of serum progesterone was high and low, respectively. We found a direct correlation between concentration of progesterone in serum and uterine content of PGE and an inverse correlation with uterine PGF2 alpha. On day 5, when progesterone was high in serum, PGE increased in tissue more than in the incubation medium, suggesting a poor liberation of this PG or utilization of PGE by the tissue. On this day, no difference was seen between content of uterine PGF2 alpha and PGF2 alpha in the incubation medium. Nevertheless, on day 10, when progesterone was lower, concentrations of both PGE and PGF2 alpha were greater (P < 0.001) in the medium than in the uterine tissue. However, PGF2 alpha in the medium was higher than PGE (P < 0.05). The results clearly demonstrate that the synthesis and release of PGE and PGF2 alpha were modified by the concentration of progesterone in the serum.