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Cycle sequencing through GC-rich regions.

作者信息

Hengen P N

机构信息

National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

出版信息

Trends Biochem Sci. 1996 Jan;21(1):33-4.

PMID:8848838
Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a number of methods used to sequence through GC-rich sections of DNA, and why it is preferable to expose X-ray film to 32P at -70 degrees C. For details on how to partake in the newsgroup, see the accompanying box.

摘要

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