Zhou Q, Sharp P A
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Science. 1996 Oct 25;274(5287):605-10. doi: 10.1126/science.274.5287.605.
Tat may stimulate transcriptional elongation by recruitment of a complex containing Tat-SF1 and a kinase to the human immunodeficiency virus-type 1 (HIV-1) promoter through a Tat-TAR interaction. A complementary DNA for the cellular activity, Tat-SF1, has been isolated. This factor is required for Tat trans-activation and is a substrate of an associated cellular kinase. Cotransfection with the complementary DNA for Tat-SF1 specifically modulates Tat activation. Tat-SF1 contains two RNA recognition motifs and a highly acidic carboxyl-terminal half. It is distantly related to EWS and FUS/TLS, members of a family of putative transcription factors with RNA recognition motifs that are associated with sarcomas.
Tat可能通过Tat-TAR相互作用将包含Tat-SF1和一种激酶的复合物募集到人免疫缺陷病毒1型（HIV-1）启动子上，从而刺激转录延伸。已分离出细胞活性因子Tat-SF1的互补DNA。该因子是Tat反式激活所必需的，并且是相关细胞激酶的底物。与Tat-SF1的互补DNA共转染可特异性调节Tat激活。Tat-SF1包含两个RNA识别基序和一个高度酸性的羧基末端半段。它与EWS和FUS/TLS有远缘关系，EWS和FUS/TLS是一个推定转录因子家族的成员，该家族具有与肉瘤相关的RNA识别基序。
