Tervahartiala T, Konttinen Y T, INgman T, Häyrinen-Immonen R, Ding Y, Sorsa T
Department of Anatomy, University of Helsinki, Finland.
J Clin Periodontol. 1996 Feb;23(2):68-75. doi: 10.1111/j.1600-051x.1996.tb00537.x.
The presence, localization and activities of cathepsin G in gingival tissue specimens and crevicular fluid (GCF) from 9 adult periodontitis patients and 6 controls with clinically healthy periodontium were studied by use of avidinbiotin-peroxidase complex method, Western and dot blotting, and spectrophotometric activity assay. In contrast to healthy gingival tissue specimens, gingival tissue specimens collected from adult periodontitis patients contained inflammatory cells in lamina propria, beneath the oral sulcular epithelium, 10-50% of which were cathepsin G positive polymorphonuclear neutrophilic leukocytes (PMNs) and monocyte/macrophage-like cells. Cathepsin G activities were increased in adult periodontitis GCF when compared to periodontally healthy controls' GCF (p < 0.05). In adult periodontitis GCF, Western blotting disclosed free cathepsin G but also clear complexes of cathepsin G with its predominant endogenous inhibitor alpha 1-antichymotrypsin (alpha 1-ACT). The present results demonstrate that part of the cathepsin G, despite the presence of increased concentrations of alpha 1-ACT, was in an uncomplexed, free and functionally active form. Our results suggest that GCF cathepsin G reflects the disease process in adjacent inflamed gingiva and also increased host response to microbiota and/or dental plaque in the periodontitis lesions. Cathepsin G may contribute to periodontal tissue destruction directly and indirectly, via proteolytic activation of latent neutrophil procollagenase (promatrix metalloproteinase-8 [proMMP-8]).
采用抗生物素蛋白-生物素-过氧化物酶复合物法、蛋白质免疫印迹法和斑点印迹法以及分光光度活性测定法，研究了9例成人牙周炎患者和6例临床牙周健康对照者的牙龈组织标本和龈沟液（GCF）中组织蛋白酶G的存在、定位及活性。与健康牙龈组织标本不同，成人牙周炎患者采集的牙龈组织标本在固有层、口腔龈沟上皮下方含有炎症细胞，其中10%-50%为组织蛋白酶G阳性的多形核中性粒细胞（PMN）和单核细胞/巨噬细胞样细胞。与牙周健康对照者的GCF相比，成人牙周炎患者GCF中的组织蛋白酶G活性增加（p<0.05）。在成人牙周炎患者的GCF中，蛋白质免疫印迹法显示存在游离的组织蛋白酶G，同时也有组织蛋白酶G与其主要内源性抑制剂α1-抗糜蛋白酶（α1-ACT）形成的清晰复合物。目前的结果表明，尽管存在浓度升高的α1-ACT，但部分组织蛋白酶G仍处于未结合的、游离的且具有功能活性的形式。我们的结果表明，GCF中的组织蛋白酶G反映了相邻炎症牙龈中的疾病进程，也反映了牙周炎病变中宿主对微生物群和/或牙菌斑反应的增强。组织蛋白酶G可能通过潜在的中性粒细胞前胶原酶（前基质金属蛋白酶-8 [proMMP-8]）的蛋白水解激活，直接或间接导致牙周组织破坏。
