Burgaleta C, Villalba S, Ferro M T
Servicios de Hematología, Hospital Ramón y Cajal, Madrid.
Sangre (Barc). 1995 Dec;40(6):509-12.
Clonal proliferation in agar, cell maturation and cell cycle characteristics were studied on peripheral blood cells from a patient with chronic myeloid leukaemia (CML) in blast crisis. Studies were performed in normal conditions and after incubation with all-trans retinoic acid 10(-6) M. At the time of the study the patients showed 300 x 10(9)/leukocytes/L (40% blast and promyelocytes). Cytogenetic studies showed 90% metaphases with t (9; 22) and t (18; 21). DNA index was 1.36. In agar cultures 450 CFU/2 x 10(5)/L cells, plus abnormal clusters were obtained, in the presence of conditioned media, and 115 normal CFU-GM after addition of all-trans retinoic acid 10(-6)M. Addition of retinoic acid to cellular suspension in liquid cultures decreased the number of immature cells from 20 to 2% in 5 days and decreased the number of cells in "S" phase from 33 to 11% after 8 days. These results show cytodinamic abnormalities in patients with CML in blast crisis and potential reversibility of these alterations by all-trans retinoic acid.
对一名处于急变期的慢性髓性白血病（CML）患者的外周血细胞进行了琼脂中克隆增殖、细胞成熟及细胞周期特征的研究。研究在正常条件下以及与10⁻⁶ M全反式维甲酸孵育后进行。研究时，患者白细胞计数为300×10⁹/L（40%为原始细胞和早幼粒细胞）。细胞遗传学研究显示90%的中期细胞具有t（9；22）和t（18；21）。DNA指数为1.36。在琼脂培养中，在有条件培养基存在的情况下，每2×10⁵/L细胞可获得450个集落形成单位（CFU）以及异常集落，添加10⁻⁶ M全反式维甲酸后可获得115个正常的粒-巨噬细胞集落形成单位（CFU-GM）。在液体培养中向细胞悬液中添加维甲酸，5天内未成熟细胞数量从20%降至2%，8天后处于“S”期的细胞数量从33%降至11%。这些结果显示了处于急变期的CML患者的细胞动力学异常以及全反式维甲酸对这些改变的潜在可逆性作用。
