Meister E F, Behrendt W, Pustowoit B, Schieck H
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde.
Laryngorhinootologie. 1996 Jan;75(1):34-7. doi: 10.1055/s-2007-997528.
We investigated the utility of cytologic studies (including follow-up studies) in our specialty in determining the prevalence of human papillomavirus, specifically in the larynx. We utilized Papanicolaou's method of exfoliative cytology and in situ hybridization of the culture (biotin-marked DNA probes).
486 patients with chronic hyperplastic laryngitis and 74 patients with papilloma were enrolled in the study.
In 198 typings in 132 patients (59 patients with papilloma, 41 with chronic hyperplastic laryngitis, 17 with cancer, and 15 healthy), we found distributions of the individual types that were independent of the diagnosis. Particularly often, we encountered human papillomavirus types 18 and 31 in the presence of dysplasia, human papillomavirus type 16 in the presence of cancer, and type 11 in general.
Cytologic examination and human papillomavirus typing of the culture permit us to define a third risk category in addition to the known risk groups smokers and dysplasia patients. Besides this, cytologic follow-up studies can provide further insight into the behavior of preneoplastic epithelial changes.
我们研究了细胞学研究（包括随访研究）在本专业中确定人乳头瘤病毒流行情况的实用性，特别是在喉部。我们采用了巴氏脱落细胞学方法和培养物原位杂交（生物素标记的DNA探针）。
486例慢性增生性喉炎患者和74例乳头状瘤患者纳入本研究。
在132例患者的198次分型中（59例乳头状瘤患者、41例慢性增生性喉炎患者、17例癌症患者和15例健康者），我们发现各型分布与诊断无关。特别是，在发育异常时我们经常遇到18型和31型人乳头瘤病毒，在癌症患者中遇到16型人乳头瘤病毒，而一般情况下为11型。
细胞学检查和培养物的人乳头瘤病毒分型使我们能够在已知的风险组吸烟者和发育异常患者之外定义第三种风险类别。除此之外，细胞学随访研究可以进一步深入了解癌前上皮变化的行为。
