Akazai A, Oda M, Nishiuchi R, Horiuchi T, Henmi M, Manki A, Seino Y
Department of Pediatrics, Okayama University School of Medicine.
Rinsho Ketsueki. 1996 Feb;37(2):95-100.
The function of IgG antibody with regard to L-asparaginase (L-asp) was investigated in vivo. Blood samples were collected before, during and after IV administration of L-asp (6,000 U/sqm for 10 days) in 18 children with acute lymphoblastic leukemia (ALL) previously treated with L-asp. Using enzyme-linked immunosorbent assay (ELISA), serum levels of L-asp and anti-L-asp IgG antibody were measured simultaneously. In 11 cases, the level of anti-L-asp IgG antibody increased prior to, but decreased to within the normal range after drug administration whereas the level of serum L-asp increased after drug administration. In 5 cases, the level of anti-L-asp IgG antibody increased as the level of serum L-asp decreased after drug administration. In contrast, in the 13 cases with no increase in anti-L-asp IgG antibody during L-asp administration, the serum L-asp level was stable. These data indicate that anti-L-asp IgG antibodies play an important role in the immunoclearance of L-asp. We would like to continue to carefully follow patients showing high titers of anti-L-asp IgG antibody during the administration of L-asp.
在体内研究了IgG抗体对L-天冬酰胺酶（L-asp）的作用。收集了18例先前接受过L-asp治疗的急性淋巴细胞白血病（ALL）儿童在静脉注射L-asp（6000 U/sqm，共10天）之前、期间和之后的血样。采用酶联免疫吸附测定（ELISA）法同时检测血清中L-asp和抗L-asp IgG抗体的水平。11例中，抗L-asp IgG抗体水平在给药前升高，但给药后降至正常范围内，而血清L-asp水平在给药后升高。5例中，给药后随着血清L-asp水平下降，抗L-asp IgG抗体水平升高。相反，在13例L-asp给药期间抗L-asp IgG抗体未升高的病例中，血清L-asp水平稳定。这些数据表明，抗L-asp IgG抗体在L-asp的免疫清除中起重要作用。我们将继续密切关注在L-asp给药期间抗L-asp IgG抗体滴度高的患者。
