Kempkes M, Wiebel F A, Golka K, Heitmann P, Bolt H M
Institut für Arbeitsphysiologie, Toxikologie und Arbeitsmedizin, Dortmund, Germany.
Arch Toxicol. 1996;70(5):306-9. doi: 10.1007/s002040050278.
Only limited information is available so far concerning the human glutathione S-transferase isoenzyme class theta encoded by the GSTT1 gene. The aim of the study was to characterize individuals in respect to a polymorphic deletion of the GSTT1 gene and to validate these results with the phenotypical determination of the "conjugator status" according to Hallier et al. (1993). Determination of the GSTT1 genotype was done in 40 healthy adults by using an assay based on internal standard controlled polymerase chain reaction. The GSTT1-1 phenotype was determined by measuring the erythrocyte conjugating activity towards methyl chloride using a gas chromatographic assay. Genotypically, 34 individuals out of 40 were classified as GSTT1 positive; the remainder were negative. These results could be confirmed by phenotyping in all but one case. In the present study the frequency of "nonconjugators" was 15%. Our study demonstrates the reliability of the suggested PCR assay for GSTT1 genotyping which is easier to perform than the phenotyping assay and is not affected by confounding factors.
迄今为止，关于由GSTT1基因编码的人类谷胱甘肽S-转移酶同工酶θ类的信息有限。本研究的目的是根据Hallier等人（1993年）的方法，对GSTT1基因多态性缺失的个体进行特征分析，并通过“结合状态”的表型测定来验证这些结果。通过基于内标控制聚合酶链反应的检测方法，对40名健康成年人进行了GSTT1基因型的测定。采用气相色谱法，通过测量红细胞对氯甲烷的结合活性来确定GSTT1-1表型。在基因型上，40名个体中有34名被归类为GSTT1阳性；其余为阴性。除一例之外，所有这些结果均可通过表型分析得到证实。在本研究中，“非结合者”的频率为15%。我们的研究证明了所建议的用于GSTT1基因分型的PCR检测方法的可靠性，该方法比表型分析方法更易于操作，且不受混杂因素的影响。
