Blanchflower W J, Kennedy D G
Veterinary Sciences Division, Department of Agriculture, Belfast, Northern Ireland, UK.
J Chromatogr B Biomed Appl. 1996 Jan 26;675(2):225-33. doi: 10.1016/0378-4347(95)00367-3.
A method is presented for the simultaneous determination of the three ionophores, monensin, salinomycin and narasin in muscle, liver and eggs from domestic fowl. Samples are homogenised in methanol and any ionophores present are extracted into toluene-hexane. The extracts are concentrated and injected into a bench-top electrospray LC-MS system. Chromatography is carried out using an end-capped reversed-phase column, with a mobile phase consisting a mixture of acetonitrile, methanol, tetrahydrofuran, trifluoroacetic acid and water. Using single-ion monitoring, the ionophores can be detected down to the 1 ng/g level.
本文介绍了一种同时测定家禽肌肉、肝脏和鸡蛋中莫能菌素、盐霉素和那拉菌素这三种离子载体的方法。将样品在甲醇中匀浆，存在的任何离子载体被萃取到甲苯 - 己烷中。萃取液浓缩后注入台式电喷雾液相色谱 - 质谱系统。使用封端反相柱进行色谱分离，流动相由乙腈、甲醇、四氢呋喃、三氟乙酸和水的混合物组成。采用单离子监测，可检测到低至1 ng/g水平的离子载体。
