Wang D Z, Song Q, Chen L M, Chao L, Chao J
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA.
Am J Physiol. 1996 Sep;271(3 Pt 2):F709-16. doi: 10.1152/ajprenal.1996.271.3.F709.
The tissue kallikrein-kinin system has been implicated in regulating blood pressure and electrolyte homeostasis. To understand the function of this system, we identified the expression and cellular localization of its components including tissue kallikrein, kallistatin, kininogen, and bradykinin B1 and B2 receptors in human adrenal gland. Reverse transcription-polymerase chain reaction followed by Southern blot analysis showed that these five components of this system were all expressed in human adrenal gland. In situ hybridization histochemistry with respective digoxigenin-labeled antisense riboprobes revealed localization of kallikrein transcript throughout the adrenal cortex and medulla except the zona glomerulosa, whereas kallistatin mRNA was only localized in the zona fasciculata. Low-molecular-weight kininogen and B2 receptor mRNAs were colocalized in the zona glomerulosa and zona fasciculata and also in the zona reticularis and chromaffin cells but to a lesser degree. The B1 receptor mRNA was stained in the zona fasciculata and medulla. These results show the expression and differential colocalization of the components of the tissue kallikrein-kinin system and reveal the potential action sites of this system in the adrenal gland.
组织激肽释放酶 - 激肽系统与血压调节和电解质平衡有关。为了解该系统的功能，我们确定了其组分包括组织激肽释放酶、激肽释放酶抑制蛋白、激肽原以及缓激肽B1和B2受体在人肾上腺中的表达及细胞定位。逆转录 - 聚合酶链反应后进行Southern印迹分析表明该系统的这五个组分在人肾上腺中均有表达。用各自地高辛标记的反义核糖探针进行原位杂交组织化学显示，激肽释放酶转录本除在球状带外遍布肾上腺皮质和髓质，而激肽释放酶抑制蛋白mRNA仅定位于束状带。低分子量激肽原和B2受体mRNA在球状带和束状带中共同定位，在网状带和嗜铬细胞中也有共同定位，但程度较低。B1受体mRNA在束状带和髓质中呈阳性染色。这些结果显示了组织激肽释放酶 - 激肽系统组分的表达及差异共定位，并揭示了该系统在肾上腺中的潜在作用位点。
