Elekes K, Hernádi L, Kiss T, Muneoka Y, Nássel D R
Department of Experimental Zoology, Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany, Hungary.
Acta Biol Hung. 1995;46(2-4):281-94.
Distribution of locustatachykinin-like immunoreactive (LomTKLI) and leucokinin like immunoreactive (LKLI) neurons in the central nervous system (CNS) of the pond snail, Lymnaea stagnalis was investigated and compared to that found in Helix pomatia. Occurrence of LomTKLI neurons in different ganglia of the freshwater bivalve, Anodonta cygnea, was also studied. Similar to Helix, the Lymnaea CNS contained LomTKLI and LKLI neurons mainly in the cerebral and pedal ganglia, but the number of labelled neurons was found to be significantly lower in Lymnaea (150-184 LomTKLI and 86-104 LKLI neurons). LomTKLI elements in anodonta ganglia were mainly confined to the neuropil, whereas the immunostained perikarya were only randomly distributed. LomTKLI and LKLI neurons were also demonstrated in a similar pattern of distribution in the intestine of Lymnaea and Helix. Analyzing the membrane effects of locustatachykinin-I, leucokinin-I and anodontatachykinin, Helix neurons were found to be either depolarized or hyperpolarized. Voltage-clamp experiments revealed the role of Ca- or K-currents in peptide effects. Our results indicate that the different tachykinin- and leucokinin-systems are involved in different central and peripheral regulatory processes of the molluscan nervous system.
研究了椎实螺（Lymnaea stagnalis）中枢神经系统（CNS）中类蝗虫速激肽免疫反应性（LomTKLI）和类亮氨酸激肽免疫反应性（LKLI）神经元的分布，并与在苹果螺（Helix pomatia）中发现的分布进行了比较。还研究了淡水双壳贝类圆田螺（Anodonta cygnea）不同神经节中LomTKLI神经元的出现情况。与苹果螺相似，椎实螺的中枢神经系统中，LomTKLI和LKLI神经元主要存在于脑和足神经节中，但发现椎实螺中标记神经元的数量明显较少（150 - 184个LomTKLI神经元和86 - 104个LKLI神经元）。圆田螺神经节中的LomTKLI成分主要局限于神经纤维网，而免疫染色的胞体只是随机分布。在椎实螺和苹果螺的肠道中也观察到LomTKLI和LKLI神经元呈现相似的分布模式。通过分析蝗虫速激肽-I、亮氨酸激肽-I和圆田螺速激肽对细胞膜的影响，发现苹果螺神经元要么去极化要么超极化。电压钳实验揭示了钙电流或钾电流在肽效应中的作用。我们的结果表明，不同的速激肽和亮氨酸激肽系统参与了软体动物神经系统不同的中枢和外周调节过程。
