Bonde M, Fledelius C, Qvist P, Christiansen C
Osteometer BioTech A/S, Herlev, Denmark.
Clin Chem. 1996 Oct;42(10):1639-44.
We present a coated-tube RIA that is useful for assessment of bone resorption. The assay uses a monoclonal antibody raised against a linear 8-amino-acid sequence (EKAHDGGR) derived from the C-telopeptides of type I collagen. Within-run and total CVs were 4.4% and 5.3-6.2%, respectively, at concentrations of 1-7 mg/L (n = 4-20). Analytical recovery was 98% +/- 8% and dilution 97% +/- 7%. Values obtained in a group of 36 premenopausal women were 227 +/- 89.6 mg/mol creatinine. In a group of 141 postmenopausal women, the values obtained were 429 +/- 225 mg/mol creatinine, a highly significant increase of 89% (P <0.001) over the premenopausal value. In a double-blind placebo-controlled clinical study of these postmenopausal women receiving five different doses of a bisphosphonate, a significant decrease of RIA-measured C-telopeptide values was seen in all bisphosphonate-treated groups, after just 3 months. Values in urine samples from postmenopausal women assayed with the RIA (gamma) and the CrossLaps(TM) ELISA (x) agreed well: slope = 0.98 (95% confidence interval, 0.94-1.01), intercept = 0.34 (0.25-0.43) mg/L, and Sylx = 0.93 mg/L (n = 678). We conclude that this RIA represents a valuable tool for assessing bone resorption.
我们介绍一种用于评估骨吸收的包被管放射免疫分析方法。该分析采用针对源自I型胶原C端肽的线性8氨基酸序列（EKAHDGGR）产生的单克隆抗体。在1 - 7mg/L浓度下（n = 4 - 20），批内变异系数和总变异系数分别为4.4%和5.3 - 6.2%。分析回收率为98%±8%，稀释回收率为97%±7%。一组36名绝经前女性测得的值为227±89.6mg/mol肌酐。在一组141名绝经后女性中，测得的值为429±225mg/mol肌酐，比绝经前值显著增加89%（P <0.001）。在一项针对这些绝经后女性接受五种不同剂量双膦酸盐的双盲安慰剂对照临床研究中，仅3个月后，所有双膦酸盐治疗组中放射免疫分析测得的C端肽值均显著下降。用放射免疫分析（γ）和CrossLaps™酶联免疫吸附测定法（x）检测的绝经后女性尿液样本中的值一致性良好：斜率 = 0.98（95%置信区间，0.94 - 1.01），截距 = 0.34（0.25 - 0.43）mg/L，且Sylx = 0.93mg/L（n = 678）。我们得出结论，这种放射免疫分析是评估骨吸收的一种有价值的工具。
