Bonde M, Qvist P, Fledelius C, Riis B J, Christiansen C
Center for Clinical and Basic Research, Ballerup, Denmark.
Clin Chem. 1994 Nov;40(11 Pt 1):2022-5.
An enzyme-linked immunosorbent assay (ELISA) for measuring type I collagen degradation products in urine < 3 h was evaluated. The measuring range was 0.5-10.5 mg/L with a detection limit of 0.2 mg/L. Within-run and total CVs were 5.3% and 6.6%, respectively. Analytical recovery averaged 100%. The mean (+/- SD) concentrations in urine samples from a healthy premenopausal population (n = 102) were 250 +/- 110 mg/mol creatinine (Cr). A group of healthy postmenopausal women (n = 410) gave a mean value of 416 +/- 189 mg/mol Cr. Values obtained in the ELISA correlated well (r = 0.83) to HPLC values for the established bone resorption marker deoxypyridinoline (n = 214), slightly better than the correlation to hydroxyproline measurements (r = 0.78, n = 421). We conclude that the assay described here presents a useful tool for further elucidating the importance of type I collagen degradation products in urine.
对一种用于测量尿液中3小时内I型胶原降解产物的酶联免疫吸附测定（ELISA）进行了评估。测量范围为0.5 - 10.5 mg/L，检测限为0.2 mg/L。批内和总变异系数分别为5.3%和6.6%。分析回收率平均为100%。健康绝经前人群（n = 102）尿液样本中的平均（±标准差）浓度为250 ± 110 mg/mol肌酐（Cr）。一组健康绝经后女性（n = 410）的平均值为416 ± 189 mg/mol Cr。ELISA获得的值与既定骨吸收标志物脱氧吡啶啉的高效液相色谱法（HPLC）值相关性良好（r = 0.83，n = 214），略优于与羟脯氨酸测量值的相关性（r = 0.78，n = 421）。我们得出结论，本文所述的测定方法是进一步阐明尿液中I型胶原降解产物重要性的有用工具。
