Sud R, Talbot I C, Delhanty J D
Human Genetics Group, Galton Laboratory, University College London, UK.
Br J Cancer. 1996 Oct;74(7):1104-8. doi: 10.1038/bjc.1996.497.
We examined 26 gastric carcinomas from British patients for mutations of the APC gene using a single-strand conformation polymorphism (SSCP) and heteroduplex assay in conjunction with the protein truncation test (PTT). In addition, we performed loss of heterozygosity (LOH) analysis of the APC and MCC genes. We detected an inactivating somatic mutation in one gastric tumour. LOH of APC was observed in one of 12 informative cases (8%) and of MCC in two of 20 cases (10%). We thus find that alteration of the APC and MCC genes are infrequent in gastric cancers from the British population. Tumour-suppressor genes on other chromosomes must play a more significant role in the development of these tumours.
我们使用单链构象多态性（SSCP）和异源双链分析结合蛋白质截短试验（PTT），对26例英国患者的胃癌进行了APC基因突变检测。此外，我们还对APC和MCC基因进行了杂合性缺失（LOH）分析。我们在一个胃肿瘤中检测到一个失活性体细胞突变。在12例信息充分的病例中，有1例（8%）观察到APC基因杂合性缺失，在20例病例中有2例（10%）观察到MCC基因杂合性缺失。因此，我们发现英国人群胃癌中APC和MCC基因的改变并不常见。其他染色体上的肿瘤抑制基因在这些肿瘤的发生发展中必定起着更重要的作用。
