Marin Castaño M E, Praddaude F, Bompart G, Girolami J P, Bascands J L
INSERM U388 Institut Louis Bugnard, CHU Rangueil, Toulouse, France.
Immunopharmacology. 1996 Jun;33(1-3):171-3. doi: 10.1016/0162-3109(96)00014-8.
Microlocalization of mRNA coding for the bradykinin 2 (BK2) receptor was carried out in the rat kidney. We combined reverse transcription and polymerase chain reaction (RT-PCR) with microdissection of individual renal tubule segments. Relative quantitation of the resulting amplified cDNA utilized densitometry of autoradiograms from Southern blots probed with a specific 32P labeled probe. The largest signals for BK2 receptor PCR product were detected in the cortical collecting tubule (CCT) and the proximal straight tubule (PST). BK2 receptor mRNA expression was also detected in the glomerulus, inner medullary thin limb (IMTL) and in the distal tubule (DT). Small but detectable signals were founded in the medullary thick ascending limb (MTAL). This distribution is consistent with multiple sites of BK action to account for different renal effects such as hemodynamics, water and electrolytic balance regulations.
对大鼠肾脏中编码缓激肽2（BK2）受体的mRNA进行了微定位研究。我们将逆转录聚合酶链反应（RT-PCR）与单个肾小管节段的显微切割相结合。利用用特定的32P标记探针杂交的Southern印迹放射自显影片的光密度测定法对所得扩增的cDNA进行相对定量。在皮质集合管（CCT）和近端直小管（PST）中检测到BK2受体PCR产物的最大信号。在肾小球、髓质内细段（IMTL）和远端小管（DT）中也检测到BK2受体mRNA表达。在髓质厚升支（MTAL）中发现了小但可检测到的信号。这种分布与BK发挥多种作用的多个位点一致，这些作用可解释不同的肾脏效应，如血流动力学、水和电解质平衡调节。
