Watanabe K, Enokihara H, Saito K, Furusawa S
Third Department of Internal Medicine, Dokkyo University School of Medicine.
Arerugi. 1995 Nov;44(11):1317-21.
To explore the pathophysiology of patients with reactive eosinophilia from unknown cause, we measured the eosinophil colony stimulating factor (Eo-CSF) activity in the interleukin-2 (IL-2) stimulated lymphocyte conditioned medium (CM) prepared from 22 patients with reactive eosinophilia. Eo-CSF activity, the levels of interleukin-5 (IL-5) and granulocyte-macrophage colony stimulating factor (GM-CSF) were increased in the CM from patients with high IgE levels. Hydrocortisone decreased the level of Elo-CSF in the CM. Elevated serum levels of soluble IL-2 receptor (sIL-2R) were presented in 13 out of 15 patients with eosinophilia. The sIL-2R levels in patients with marked eosinophilia (>3000/mu l) were higher than those in patients with mild eosinophilia (< or = 3000/mu l). High sIL-2R levels were noted in T cell CM from 3 out of 15 patients and in eosinophil CM from 1 out of 4 patients. These data suggested that lymphocyte from eosinophilic patients with elevated IgE produce Eo-CSF, IL-5 and GM-CSF by IL-2 stimulation. Eo-CSF production is inhibited by hydrocortisone. SIL-2R is released from lymphocyte and in some case may be released from eosinophils.
为探讨不明原因反应性嗜酸性粒细胞增多症患者的病理生理学，我们检测了22例反应性嗜酸性粒细胞增多症患者经白细胞介素-2（IL-2）刺激的淋巴细胞条件培养基（CM）中的嗜酸性粒细胞集落刺激因子（Eo-CSF）活性。高IgE水平患者的CM中Eo-CSF活性、白细胞介素-5（IL-5）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）水平升高。氢化可的松降低了CM中Elo-CSF的水平。15例嗜酸性粒细胞增多症患者中有13例血清可溶性IL-2受体（sIL-2R）水平升高。嗜酸性粒细胞显著增多（>3000/μl）的患者的sIL-2R水平高于轻度嗜酸性粒细胞增多（≤3000/μl）的患者。15例患者中有3例的T细胞CM和4例患者中有1例的嗜酸性粒细胞CM中sIL-2R水平较高。这些数据表明，IgE升高的嗜酸性粒细胞增多症患者的淋巴细胞经IL-2刺激可产生Eo-CSF、IL-5和GM-CSF。氢化可的松可抑制Eo-CSF的产生。SIL-2R从淋巴细胞释放，在某些情况下可能从嗜酸性粒细胞释放。
