Martin M R, Caddy K W, Biscoe T J
J Anat. 1977 Jul;123(Pt 3):579-87.
The number and diameter of the motoneurons in serial sections of the facial nucleus in the albino rat were estimated using a photograph-camera lucida technique for counting and measuring cells in conjunction with a systematic section method for sampling the population. The mean diameter of facial motoneuron nucleoli was also estimated. Total cell counts were estimated using two formulas, one a basic count and the other the basic count corrected for split nucleoli errors. The mean motoneuron diameter is 33-93 micronm (+/- 6-18 micronm S.D.). The number and diameter of myelinated axons in both the facial nerve and its cutaneous auricular branch at the level of the stylomastoid foramen were estimated. There are 5353 myelinated axons in the facial nerve, with a mean diameter of 2-74 micronm, and 627 myelinated axons in the cutaneous auricular branch with a mean diameter of 1-47 micronm. The accuracy and reproductibility of results using the photograph-camera lucida technique for counting and measuring cells and the systematic section method of sampling are compared with those of the methods used by previous investigators. The accuracy of results obtained for estimates of the total cell count using the formulas for the basic count and the basic count corrected for split nucleoli are also compared.
利用照相目镜描绘器技术对细胞进行计数和测量，并结合系统切片法对群体进行抽样，对白化大鼠面神经核连续切片中的运动神经元数量和直径进行了估计。还对面部运动神经元核仁的平均直径进行了估计。使用两个公式估计细胞总数，一个是基本计数，另一个是针对分裂核仁误差校正后的基本计数。运动神经元的平均直径为33 - 93微米（标准差为±6 - 18微米）。对茎乳孔水平的面神经及其耳颞支中的有髓轴突数量和直径进行了估计。面神经中有5353条有髓轴突，平均直径为2 - 74微米，耳颞支中有627条有髓轴突，平均直径为1 - 47微米。将使用照相目镜描绘器技术对细胞进行计数和测量以及系统切片抽样方法所得到结果的准确性和可重复性，与先前研究者使用的方法进行了比较。还比较了使用基本计数公式和针对分裂核仁校正后的基本计数公式对细胞总数估计所得到结果的准确性。
