Kamath K R, Danilich M J, Marchant R E, Park K
Purdue University, School of Pharmacy, West Lafayette, IN 47907, USA.
J Biomater Sci Polym Ed. 1996;7(11):977-88. doi: 10.1163/156856296x00381.
Dimethyldichlorosilane (DDS)-treated glass (DDS-glass) was modified with either poly(ethylene oxide) (PEO) films or poly(N-vinyl-2-pyrrolidone) (PNVP) films by plasma polymerization. The thickness of the plasma polymerized films was varied between 40 and 700 nm. The results showed that the hydrophilic plasma polymerized PEO and PNVP films on DDS-glass did not prevent platelet adhesion and activation. The film thickness had only marginal influence on the prevention of platelet activation. In contrast, platelet adhesion was prevented on DDS-glass absorbed with a PEO-containing block copolymer (Pluronic F-108 surfactant) even at a calculated thickness of the PEO layer of less than 40 nm. This study shows that surface hydrophilization is not sufficient for prevention of platelet adhesion and activation. The contrasting results in platelet adhesion between cross-linked plasma polymers and linear PEO-containing block copolymers may be explained qualitatively by a steric repulsion mechanism that is achieved by the conformational freedom of the linear PEO chains interacting with water.
通过等离子体聚合，用聚环氧乙烷（PEO）薄膜或聚N - 乙烯基 - 2 - 吡咯烷酮（PNVP）薄膜对二甲基二氯硅烷（DDS）处理过的玻璃（DDS - 玻璃）进行改性。等离子体聚合薄膜的厚度在40至700纳米之间变化。结果表明，DDS - 玻璃上亲水性的等离子体聚合PEO和PNVP薄膜不能阻止血小板的黏附和活化。薄膜厚度对防止血小板活化仅有微小影响。相比之下，即使在计算得出的PEO层厚度小于40纳米时，用含PEO的嵌段共聚物（普朗尼克F - 108表面活性剂）吸收的DDS - 玻璃上也能防止血小板黏附。本研究表明，表面亲水化不足以防止血小板黏附和活化。交联等离子体聚合物与含线性PEO的嵌段共聚物在血小板黏附方面的对比结果，可能通过空间排斥机制进行定性解释，该机制是由线性PEO链与水相互作用的构象自由度实现的。
