Rao P V, Bhaskar A S, Bhattacharya R
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Microbios. 1996;86(347):95-104.
The effects of media and culture duration on growth, macromolecular composition and toxicity of the freshwater cyanobacterium Microcystis aeruginosa were evaluated. Four media (MA, CB, A3M7 and BG-11) influenced growth in terms of cell numbers, chlorophyll a content and specific growth rate. MA medium supported the best growth. The macromolecular composition of cultured cells, viz total protein, carbohydrates, lipids, DNA and RNA content, varied reaching a maximum at various growth periods. The differences were significant due to the interaction of the culture medium and duration. Toxicity was compared in terms of mouse units. The toxicity of cultured cells increased with the duration until week 4 and toxicity was highest in cells grown in MA medium.
评估了培养基和培养时间对淡水蓝藻铜绿微囊藻生长、大分子组成及毒性的影响。四种培养基（MA、CB、A3M7和BG - 11）在细胞数量、叶绿素a含量和比生长速率方面影响生长。MA培养基支持最佳生长。培养细胞的大分子组成，即总蛋白、碳水化合物、脂质、DNA和RNA含量，各不相同，在不同生长时期达到最大值。由于培养基和培养时间的相互作用，差异显著。以小鼠单位比较毒性。培养细胞的毒性随培养时间增加直至第4周，且在MA培养基中生长的细胞毒性最高。
