Sherline P, Mundy G R
J Cell Biol. 1977 Aug;74(2):371-6. doi: 10.1083/jcb.74.2.371.
The role of the tubulin-microtubule system was examined in human peripheral blood leukocytes after activation with phytohemagglutinin (PHA). Soluble tubulin and microtubules were measured with a [(3)H]colchicine-binding assay. It was found that the tubulin content of PHA-activated lymphocytes was consistently increased relative to total protein content after 36 h of culture. There was no increase in the proportion of total tubulin synthesis which was present as microtubules at 36 h. Nevertheless, as a result of increased tubulin synthesis, there was a two-to three-fold increase in total microtubular mass. Colchicine, which disrupts microtubles, was used to assess the role of microtubule assembly in the sequence of events which follow lymphocyte activation, namely lymphokine release, protein synthesis, RNA synthesis, and DNA synthesis. Colchicine consistently inhibited DNA synthesis but did not inhibit release of the lymphokine, osteoclast activating factor (OAF). Protein and RNA syntheses were inhibited much less than DNA synthesis. The fact that some effects of PHA on lymphocytes appear to require intact microtubules and at least one does not suggest that the microtubule dependent step in PHA-stimulated lymphocyte activation occurs at a stage after propagation of the signal from the membrane to the cell interior.
在用植物血凝素（PHA）激活后，研究了微管蛋白 - 微管系统在人外周血白细胞中的作用。用[³H]秋水仙碱结合试验测定可溶性微管蛋白和微管。结果发现，培养36小时后，PHA激活的淋巴细胞中微管蛋白含量相对于总蛋白含量持续增加。36小时时，作为微管存在的总微管蛋白合成比例没有增加。然而，由于微管蛋白合成增加，微管总质量增加了两到三倍。破坏微管的秋水仙碱用于评估微管组装在淋巴细胞激活后一系列事件中的作用，这些事件包括淋巴因子释放、蛋白质合成、RNA合成和DNA合成。秋水仙碱持续抑制DNA合成，但不抑制破骨细胞激活因子（OAF）这一淋巴因子的释放。蛋白质和RNA合成受到的抑制远小于DNA合成。PHA对淋巴细胞的某些作用似乎需要完整的微管，而至少有一种作用并非如此，这表明PHA刺激的淋巴细胞激活中依赖微管的步骤发生在信号从膜传递到细胞内部之后的阶段。
