正常及白血病淋巴细胞的肌动蛋白和微管蛋白
Actin and tubulin of normal and leukaemic lymphocytes.
作者信息
Atkins H, Anderson P J
出版信息
Biochem J. 1982 Dec 1;207(3):535-9. doi: 10.1042/bj2070535.
Double labelling and the isolation of actin- and tubulin-derived peptides were used to determine the amounts of these proteins in peripheral lymphocytes from normal donors and from patients with chronic lymphocytic leukaemia. As a precaution against proteolysis, samples were boiled before assay. The actin content of chronic-lymphocytic-leukaemia (CLL) lymphocytes was 8.1 +/- 2.1% of total protein, which was lower (P less than 0.05) than the amount (12.8 +/- 3.0%) of actin found in normal lymphocytes. The tubulin content of CLL lymphocytes was 4.4 +/- 1.5% of total protein, which was also significantly less (P less than 0.05) than that of normal lymphocytes, which was found to be 6.1 +/- 1.1%.
采用双重标记法以及肌动蛋白和微管蛋白衍生肽的分离方法，来测定正常供体和慢性淋巴细胞白血病患者外周血淋巴细胞中这些蛋白质的含量。作为防止蛋白水解的一项预防措施，样品在检测前进行煮沸。慢性淋巴细胞白血病（CLL）淋巴细胞的肌动蛋白含量占总蛋白的8.1±2.1%，低于正常淋巴细胞中肌动蛋白的含量（12.8±3.0%）（P<0.05）。CLL淋巴细胞的微管蛋白含量占总蛋白的4.4±1.5%，同样显著低于正常淋巴细胞（6.1±1.1%）（P<0.05）。
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