Dorey C K, Aouididi S, Reynaud X, Dvorak H F, Brown L F
Schepens Eye Research Institute, Beth Israel Hospital, Harvard Medical School, Boston, Mass, USA.
Arch Ophthalmol. 1996 Oct;114(10):1210-7. doi: 10.1001/archopht.1996.01100140410008.
To determine whether neovascularization was spatially correlated with the distribution of messenger RNA for vascular permeability factor/vascular endothelial growth factor (VPF/VEGF).
Neonatal rats were raised 8 days in 80% oxygen with daily intervals of relative hypoxia in room air, then transferred to room air for 5, 7, or 10 additional days. In situ hybridization for VPF/VEGF expression and avascular area were examined in retinas from oxygen-exposed animals and room-air controls. Severity of neovascularization was scored.
The inner nuclear layer of oxygen-exposed retinas exhibited a continuous intense band of VPF/VEGF messenger RNA expression across the peripheral avascular zone that dropped sharply in vascular retina. Neovascularization occurred adjacent to regions of greatest expression. Controls had much lower expression and smaller avascular regions. The VPF/VEGF messenger RNA expression was most intense in Müller cells, scattered astrocytes, and amacrine cells, strong in retinal pigment epithelium, and moderate in the remaining inner nuclear layer and ganglion cell layer.
The expression of VPF/VEGF message was spatially and quantitatively correlated with the neovascularization.
确定新生血管形成是否与血管通透因子/血管内皮生长因子（VPF/VEGF）信使核糖核酸的分布在空间上相关。
将新生大鼠置于80%氧气环境中饲养8天，期间每天间隔置于室内空气中相对缺氧环境，然后再转移至室内空气环境中额外饲养5、7或10天。对暴露于氧气环境的动物和室内空气对照动物的视网膜进行VPF/VEGF表达的原位杂交以及无血管区域检查。对新生血管形成的严重程度进行评分。
暴露于氧气环境的视网膜内核层在周边无血管区呈现出一条连续强烈的VPF/VEGF信使核糖核酸表达带，在有血管的视网膜中急剧下降。新生血管形成发生在表达最强烈的区域附近。对照组的表达水平低得多，无血管区域也较小。VPF/VEGF信使核糖核酸在穆勒细胞、散在的星形胶质细胞和无长突细胞中表达最强烈，在视网膜色素上皮中表达较强，在其余内核层和神经节细胞层中表达中等。
VPF/VEGF信息的表达在空间和数量上与新生血管形成相关。
