Byrd P, Wise D, Dentler W L
Department of Biological Sciences, Mississippi State University, Starkville, USA.
Cell Motil Cytoskeleton. 1996;34(1):57-68. doi: 10.1002/(SICI)1097-0169(1996)34:1<57::AID-CM6>3.0.CO;2-F.
Cell division was arrested by injection of a preimmune rabbit serum, B-61, into PtK(2) cells during interphase and prometaphase. Identical results were obtained by injection of whole B-61 antiserum and of antibodies affinity-purified from the serum against a 66-kDa PtK(2) cell polypeptide. When injected into interphase cells, the antibodies arrested further development and cell division. When injected into prometaphase and metaphase cells, spindles shortened and poles moved together at a rate of 0.2-0.4 mu m/min, approximately half the rate of anaphase A chromosome movements in normally dividing PtK(2) cells. Chromosomes decondensed and cells did not reenter division. Both whole antisera and affinity-purified antibodies stained antigens diffusely localized throughout the cytoplasm in dividing and interphase cells. These results suggest that the 66-kDa antigen is a nonspindle protein that may regulate mitotic progression in PtK(2) cells.
在间期和前中期向PtK(2)细胞中注射免疫前兔血清B - 61可使细胞分裂停滞。注射完整的B - 61抗血清以及从该血清中针对一种66 kDa PtK(2)细胞多肽亲和纯化得到的抗体，也获得了相同的结果。当注射到间期细胞中时，这些抗体阻止了细胞的进一步发育和细胞分裂。当注射到前中期和中期细胞中时，纺锤体缩短，两极以0.2 - 0.4μm/分钟的速度一起移动，大约是正常分裂的PtK(2)细胞中A期染色体移动速度的一半。染色体解聚，细胞不再进入分裂。完整抗血清和亲和纯化抗体均使抗原在分裂细胞和间期细胞的整个细胞质中呈弥漫性定位染色。这些结果表明，66 kDa抗原是一种非纺锤体蛋白，可能在PtK(2)细胞中调节有丝分裂进程。
