Riekkinen P, Mettinger K L, Karukin M, Riekkinen M
Department of Neurology, University of Kuopio, Kuopio, Finland.
Brain Res. 1996 Apr 1;714(1-2):118-24. doi: 10.1016/0006-8993(95)01522-1.
The present study investigated the effects of repeated daily administration of a mixture containing free fatty acids (alpha-linolenic/linoleic acid in a 1:4 ratio at 25 mg/kg i.p., Alzene) on medial septal (MS) lesion-induced impairment of water maze (WM) spatial reference and working memory or passive avoidance (PA) behavior in adult rats (250-275 g at the beginning of the study). Alzene treatment was started 7 days before initiation of behavioral testing (WM reference memory testing on treatment days: 7-9 and 28-30 + passive avoidance on treatment days 31-32; WM working memory testing on treatment days: 7-10 and 38-31 + passive avoidance testing on treatment days 32-33) and continued until the end of the study. Alzene improved WM reference memory (treatment days 7-9 and 28-30) and PA behavior (treatment days 31-32) as effectively as an anti-cholinesterase drug, tacrine 3 mg/kg (i.p.). However, in a separate group of MS-lesioned rats we observed that working memory (treatment days 7-10 and 28-31) was not improved by either Alzene or tacrine treatment. The present results suggest that Alzene treatment improves spatial reference memory and inhibitory avoidance in MS-lesioned rats.
本研究调查了每日重复腹腔注射含游离脂肪酸混合物（α-亚麻酸/亚油酸，比例为1:4，剂量为25mg/kg，商品名Alzene）对成年大鼠（研究开始时体重250-275g）内侧隔区（MS）损伤所致水迷宫（WM）空间参考记忆和工作记忆损害或被动回避（PA）行为的影响。在行为测试开始前7天开始给予Alzene治疗（治疗日7-9和28-30进行WM参考记忆测试+治疗日31-32进行被动回避测试；治疗日7-10和38-31进行WM工作记忆测试+治疗日32-33进行被动回避测试），并持续至研究结束。Alzene改善WM参考记忆（治疗日7-9和28-30）和PA行为（治疗日31-32）的效果与抗胆碱酯酶药物他克林3mg/kg（腹腔注射）相当。然而，在另一组MS损伤大鼠中，我们观察到Alzene和他克林治疗均未改善工作记忆（治疗日7-10和28-31）。目前的结果表明，Alzene治疗可改善MS损伤大鼠的空间参考记忆和抑制性回避。
