Jackson D J, Saunders V A, Humphreys A M
School of Biomolecular Sciences, Liverpool John Moores University, UK.
Lett Appl Microbiol. 1996 Sep;23(3):159-62. doi: 10.1111/j.1472-765x.1996.tb00054.x.
Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuole fraction contained no significant chitinase activity, but was enriched in chitinase activating factor. Activity of microsomal chitinase increased upon incubation with this, but no other gradient factor. Results suggest that the regulatory system governing microsomal chitinase activity, like that governing chitin synthase, involves a 'vacuolar' activating factor in Candida albicans.
来自白色念珠菌酵母细胞和菌丝细胞的微粒体几丁质酶在30℃孵育时可被内源性激活，也可被胰蛋白酶外源性激活。通过在碘克沙醇密度梯度上对裂解的原生质体进行分级分离，将酵母细胞的假定激活因子与几丁质酶活性分离。液泡部分没有显著的几丁质酶活性，但富含几丁质酶激活因子。微粒体几丁质酶与该因子孵育后活性增加，与其他梯度因子孵育则不然。结果表明，白色念珠菌中调控微粒体几丁质酶活性的系统，如同调控几丁质合酶的系统一样，涉及一种“液泡”激活因子。
