从大肠菌群富集培养物中检测大肠杆菌O157:H7。
Detection of Escherichia coli O157:H7 in water from coliform enrichment cultures.
作者信息
Rice E W, Johnson C H, Reasoner D J
机构信息
US Environmental Protection Agency, Cincinnati, OH 45268, USA.
出版信息
Lett Appl Microbiol. 1996 Sep;23(3):179-82.
PMID:8862024
Abstract
Environmental water samples were seeded with Escherichia coli O157:H7 and the bacterium was recovered using a traditional coliform enrichment procedure followed by selective plating on sorbitol MacConkey agar and biochemical and serological characterization. Assays for beta-glucuronidase and glutamate decarboxylase were found to be useful procedures for screening suspected isolates. The organism was not recovered in a survey of various water samples.
摘要
将大肠杆菌O157:H7接种到环境水样中，然后使用传统的大肠菌群富集程序回收该细菌，接着在山梨醇麦康凯琼脂上进行选择性平板培养，并进行生化和血清学鉴定。结果发现，β-葡萄糖醛酸酶和谷氨酸脱羧酶检测是筛选疑似分离株的有用方法。在对各种水样的调查中未回收该微生物。
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