Pender M P, Csurhes P A, Houghten R A, McCombe P A, Good M F
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Herston, Australia.
J Neuroimmunol. 1996 Oct;70(1):65-74. doi: 10.1016/s0165-5728(96)00105-1.
We generated T-cell lines from the peripheral blood of controls and of patients with multiple sclerosis (MS) by stimulation with overlapping synthetic peptides representing the entire sequences of all four isoforms of human myelin basic protein (MBP). The T-cell lines reacted to a wide range of epitopes in the major isoforms of MBP and to epitopes that were present only in the minor isoforms. Many MS patients and controls had T-cells responding to one or more cryptic MBP epitopes, as indicated by the generation of a peptide-specific T-cell line(s) by stimulation with synthetic peptides but not by stimulation with whole MBP. About one-third of the peptide-generated lines were cytotoxic. Although we have shown that this technique of peptide stimulation is effective in generating human antiviral cytotoxic CD8+ T-cell lines, all the cytotoxic MBP-specific lines generated by this method were predominantly CD4+. Our study did not reveal any significant differences, between MS patients and controls, in reactivity to epitopes within any of the isoforms of MBP.
我们通过用代表人类髓鞘碱性蛋白（MBP）所有四种亚型完整序列的重叠合成肽刺激，从对照者和多发性硬化症（MS）患者的外周血中生成了T细胞系。这些T细胞系对MBP主要亚型中的多种表位以及仅存在于次要亚型中的表位有反应。许多MS患者和对照者有T细胞对一个或多个隐蔽的MBP表位产生反应，这表现为用合成肽刺激可产生肽特异性T细胞系，而用完整MBP刺激则不能。约三分之一的由肽产生的细胞系具有细胞毒性。尽管我们已表明这种肽刺激技术在生成人类抗病毒细胞毒性CD8 + T细胞系方面是有效的，但通过这种方法产生的所有细胞毒性MBP特异性细胞系主要是CD4 +。我们的研究未揭示MS患者和对照者在对MBP任何亚型内表位的反应性方面存在任何显著差异。
