Sharkey M E, Adler R R, Nieder G L, Brenner C A
Department of Biology, Clark University, Worcester, Massachusetts, USA.
Am J Reprod Immunol. 1996 Aug;36(2):72-80. doi: 10.1111/j.1600-0897.1996.tb00142.x.
The purpose of this study was to define the temporal expression and to quantitate the mRNA levels of collagenase, 72 kDa, 92 kDa, and membrane-type matrix metalloproteinase during the peri-implantation period of pregnancy in the mouse uterus. Embryonic expression of 72 kDa and 92 kDa matrix metalloproteinases, as well as interleukin 1 alpha, was also investigated.
Uterine matrix metalloproteinases were detected using gelatin substrate gel electrophoresis (zymography) and reverse-transcription polymerase chain reaction methodology was used to detect and quantitate different mRNA species in the mouse uterus and blastocyst.
Collagenase, 72 kDa, and 92 kDa matrix metalloproteinases are developmentally regulated during the peri-implantation period of pregnancy, but membrane-type matrix metalloproteinase appears to be expressed constitutively. Matrix metalloproteinase mRNA levels have been quantitated and confirm the observed developmental expression patterns. Prominent expression of bot 92 kDa matrix metalloproteinase and interleukin 1 alpha was observed in blastocysts during outgrowth while weak expression of the 72 kDa matrix metalloproteinase was detected.
The date provide evidence of matrix metalloproteinase expression in vivo and substantiate their potential role in tissue remodeling prior to and during blastocyst implantation. Expression of interleukin 1 alpha, 92 kDa, and 72 kDa matrix metalloproteinases suggests that these proteins are important for trophoblast invasion associated with implantation of the early embryo.
本研究的目的是确定小鼠子宫妊娠植入前期胶原酶、72 kDa、92 kDa和膜型基质金属蛋白酶的时间表达并对其mRNA水平进行定量。还研究了72 kDa和92 kDa基质金属蛋白酶以及白细胞介素1α的胚胎表达情况。
使用明胶底物凝胶电泳（酶谱法）检测子宫基质金属蛋白酶，并采用逆转录聚合酶链反应方法检测和定量小鼠子宫及囊胚中不同的mRNA种类。
胶原酶、72 kDa和92 kDa基质金属蛋白酶在妊娠植入前期受到发育调控，但膜型基质金属蛋白酶似乎是组成性表达。已对基质金属蛋白酶mRNA水平进行定量，证实了观察到的发育表达模式。在囊胚生长过程中观察到92 kDa基质金属蛋白酶和白细胞介素1α均有显著表达，而72 kDa基质金属蛋白酶表达较弱。
这些数据提供了基质金属蛋白酶在体内表达的证据，并证实了它们在囊胚植入前及植入过程中组织重塑中的潜在作用。白细胞介素1α、92 kDa和72 kDa基质金属蛋白酶的表达表明，这些蛋白质对与早期胚胎植入相关的滋养层细胞侵袭很重要。
