Li P, Giddings J C
Department of Chemistry, University of Utah, Salt Lake City 84112, USA.
J Pharm Sci. 1996 Aug;85(8):895-8. doi: 10.1021/js950335s.
Using a modified flow field-flow fractionation (flow FFF) technique termed membrane-selective flow FFF, a capability is developed to isolate various colloidal constituents that are naturally present in or may be introduced into blood plasma. Once isolated, the colloids may be subject to quantitative measurement to provide relative amounts of the different constituents and the size distribution curve of each. The potential of the technique has been demonstrated by isolating and measuring (a) the lipoprotein fractions found in human blood plasma, (b) liposyn II pharmaceutical emulsion added at 2 mg/mL of plasma, and (c) amphotericin B colloidal dispersion (a drug delivery agent) added to plasma at 0.5 mg/mL.
使用一种称为膜选择性流场-流分级分离（流动 FFF）的改良技术，开发出一种能够分离血浆中天然存在或可能引入的各种胶体成分的能力。一旦分离出来，这些胶体可以进行定量测量，以提供不同成分的相对含量以及每种成分的尺寸分布曲线。通过分离和测量（a）人血浆中的脂蛋白组分、（b）以 2 mg/mL 血浆的添加量加入的 Liposyn II 药用乳剂以及（c）以 0.5 mg/mL 血浆的添加量加入血浆的两性霉素 B 胶态分散体（一种药物递送剂），证明了该技术的潜力。
