Rosengren L E, Karlsson J E, Karlsson J O, Persson L I, Wikkelsø C
Institute of Anatomy and Cell Biology, University of Göteborg, Sweden.
J Neurochem. 1996 Nov;67(5):2013-8. doi: 10.1046/j.1471-4159.1996.67052013.x.
In the present study we describe an ELISA to quantify the light subunit of the neurofilament triplet protein (NFL) in CSF. The method was validated by measuring CSF NFL concentrations in healthy individuals and in two well-characterized groups of patients with amyotrophic lateral sclerosis (ALS) and Alzheimer's disease (AD). The levels were increased in ALS (1,743 +/- 1,661 ng/L; mean +/- SD) and AD (346 +/- 176 ng/L) compared with controls (138 +/- 31 ng/L; p < 0.0001 for both). Within the ALS group, patients with lower motor neuron signs only had lower NFL levels (360 +/- 237 ng/L) than those with signs of upper motor neuron disease (2,435 +/- 1,633 ng/L) (p < 0.05). In a second study patients with miscellaneous neurodegenerative diseases were investigated (vascular dementia, olivopontocerebellar atrophy, normal pressure hydrocephalus, cerebral infarctions, and multiple sclerosis), and the CSF NFL level was found to be increased (665 +/- 385 ng/L; p < 0.0001). NFL is a main structural protein of axons, and we suggest that CSF NFL can be used to monitor neurodegeneration in general, but particularly in ALS with involvement of the pyramidal tract.
在本研究中，我们描述了一种用于定量脑脊液中神经丝三联蛋白（NFL）轻链亚基的酶联免疫吸附测定法（ELISA）。通过测量健康个体以及两组特征明确的肌萎缩侧索硬化症（ALS）和阿尔茨海默病（AD）患者脑脊液中NFL的浓度，对该方法进行了验证。与对照组（138±31 ng/L）相比，ALS组（1,743±1,661 ng/L；平均值±标准差）和AD组（346±176 ng/L）的水平均升高（两组p均<0.0001）。在ALS组中，仅出现下运动神经元体征的患者NFL水平（360±237 ng/L）低于出现上运动神经元疾病体征的患者（2,435±1,633 ng/L）（p<0.05）。在第二项研究中，对患有各种神经退行性疾病的患者（血管性痴呆、橄榄脑桥小脑萎缩、正常压力脑积水、脑梗死和多发性硬化症）进行了调查，发现脑脊液NFL水平升高（665±385 ng/L；p<0.0001）。NFL是轴突的主要结构蛋白，我们认为脑脊液NFL一般可用于监测神经退行性变，尤其是在累及锥体束的ALS中。
