Erill N, Cuatrecasas M, Sancho F J, Farré A, Pour P M, Lluís F, Capellá G
Laboratory of Gastrointestinal Investigation, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Am J Pathol. 1996 Oct;149(4):1333-9.
K-ras and p53 gene alterations are frequently found in human pancreatic carcinomas and cell lines. The aim of this study was to analyze for the presence of K-ras and p53 gene mutations in hamster pancreatic tumors and cell lines. Mutations at the first coding exon of the K-ras gene and in exons V to VIII of the hamster p53 gene were analyzed in six cell lines (H2T, PC1, PC1.2, PC1.0, WD, and PD) and in N-nitroso-bis(2-oxopropyl)amine-induced pancreatic (n = 9) and extra-pancreatic (n = 4) tumors. K-ras mutations were present in seven of the nine pancreatic tumors and in all extra-pancreatic tumors. No p53 mutations were detected in the tumors. All cell lines analyzed contained K-ras mutations. Moreover, four of the six cell lines contained single amino acid substitutions in the p53 gene. Cell lines derived from nitrosamine-induced pancreatic tumors in the hamster contained K-ras and p53 alterations similar to those found in cell lines derived from human pancreatic carcinomas.
K-ras和p53基因改变在人类胰腺癌及细胞系中经常被发现。本研究的目的是分析仓鼠胰腺肿瘤及细胞系中K-ras和p53基因突变的存在情况。在六个细胞系（H2T、PC1、PC1.2、PC1.0、WD和PD）以及N-亚硝基双（2-氧代丙基）胺诱导的胰腺肿瘤（n = 9）和胰腺外肿瘤（n = 4）中，分析了仓鼠K-ras基因的第一个编码外显子以及p53基因外显子V至VIII的突变情况。九个胰腺肿瘤中有七个存在K-ras突变，所有胰腺外肿瘤均存在K-ras突变。在肿瘤中未检测到p53突变。所有分析的细胞系均含有K-ras突变。此外，六个细胞系中有四个在p53基因中存在单氨基酸替换。源自仓鼠亚硝胺诱导的胰腺肿瘤的细胞系含有与源自人类胰腺癌的细胞系中发现的类似的K-ras和p53改变。
